Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- NB100-807 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-807, RRID:AB_2129670
- Product name
- Goat Polyclonal ITK Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K
FEBS letters 1993 Jun 7;324(1):1-5
FEBS letters 1993 Jun 7;324(1):1-5
A novel human tyrosine kinase gene inducible in T cells by interleukin 2.
Tanaka N, Asao H, Ohtani K, Nakamura M, Sugamura K
FEBS letters 1993 Jun 7;324(1):1-5
FEBS letters 1993 Jun 7;324(1):1-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ITK Antibody [NB100-807] - (2ug/ml) of Jurkat lysate (RIPA buffer, 1.4E+05 cells per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ITK Antibody [NB100-807] - HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human (ITK) with DYKDDDDK tag probed with EB05117 (1 ug/mL) in Lane A and probed with anti-DYKDDDDK Tag (1:10000) in lane C. Mock-transfected HEK293 probed with EB10338 (1 mg/mL) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ITK Antibody [NB100-807] - Staining of Jurkat nuclear cell lysate with antibody at 1 ug/mL (A) and negative control Human parathyroid gland (B) (35 ug protein in RIPA buffer). Detected by chemiluminescence.