PAB27496

antibody from Abnova Corporation

Targeting: EGR4 NGFI-C, PAT133

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB27496

Provider

Abnova Corporation

Product name

EGR4 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant EGR4.

Antigen sequence

PWELLSVGAPGNCGSQGDYQAAPEARFPVIGTKIE
DLLSISCPAELPAVPANRLYPSGAYDAFPLAPGDL
GEGAEGLPGLLTPPSGEGGSSGDGGEFLASTQPQL
SPLGLRSAAAADFPKPL


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251MG with EGR4 polyclonal antibody (Cat # PAB27496) at 1-4 ug/mL dilutions shows positivity in nucleus but not nucleoli, cytoplasm and vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human kidney with EGR4 polyclonal antibody (Cat # PAB27496) shows strong cytoplasmic positivity in tubular cells at 1:50-1:200 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)