Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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Validation data
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- Product number
- MA5-34892 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UAP1 Monoclonal Antibody (4H1)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- Positive Control: Jurkat, A549, Hela, rat epididymis tissue, human lung cancer tissue, human colon cancer tissue, mouse testis tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 4H1
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UAP1 in Jurkat cell lysate. Samples were incubated with UAP1 monoclonal antibody (Product # MA5-34892), at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UAP1 in HeLa cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UAP1 monoclonal antibody (Product # MA5-34892), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UAP1 in A549 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with UAP1 monoclonal antibody (Product # MA5-34892), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UAP1 in paraffin-embedded rat epididymis tissue. Samples were incubated with UAP1 monoclonal antibody (Product # MA5-34892), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UAP1 in paraffin-embedded mouse testis tissue. Samples were incubated with UAP1 monoclonal antibody (Product # MA5-34892), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UAP1 in paraffin-embedded human lung cancer tissue. Samples were incubated with UAP1 monoclonal antibody (Product # MA5-34892), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of UAP1 in paraffin-embedded human colon cancer tissue. Samples were incubated with UAP1 monoclonal antibody (Product # MA5-34892), and followed by hematoxylin.