Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UAP1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using UAP1 Polyclonal Antibody (Product # PA5-21500). Sample (30 µg of whole cell lysate). A: H1299. 7.5% SDS PAGE. UAP1 Polyclonal Antibody (Product # PA5-21500) diluted at 1:3,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of UAP1 in paraformaldehyde-fixed A549 cells using a UAP1 polyclonal antibody (Product # PA5-21500) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of UAP1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: UAP1 Polyclonal Antibody (Product # PA5-21500) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using UAP1 antibody (Product # PA5-21500) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES-2 xenograft , using UAP1 (Product # PA5-21500) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.