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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- LS-C745415 - Provider product page
- Provider
- LSBio
- Product name
- O3FAR1 / GPR120 Antibody (N-Terminus) LS-C745415
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western Blot of rabbit Anti-Ffar4 antibody. Lane 1: Ladder (Opal Prestained) p/n MB-210-0500. Lane 2: Raw 264.7 WCL p/n W10-001-369. Lane 3: NIH/3T3 WCL p/n W10-000-358. Lane 4: PC-12 WCL p/n W12-001-GL9. Lane 5: Ladder (Opal Prestained) p/n MB-210-0500. Load: 35 µg per lane. Primary antibody: Ffar4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Gt-a-Rb HRP secondary antibody 611-103-122 at 1:70,000 for 30 min at RT. Block: MB-073 BlockOut Universal Blocking Buffer for 30 min at RT. Predicted/Observed size: 40.8 kDa, observed ~48 kDa band due to glycosylation of Ffar4 protein.
