AFR-014-25UL

antibody from Invitrogen Antibodies

Targeting: FFAR4 GPR120, GPR129, O3FAR1, PGR4

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AFR-014-25UL

Provider

Invitrogen Antibodies

Product name

GPR120/FFAR4 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse colon (lanes 9 and 11) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 10 and 12) lysates: - 9,10. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).11,12. Anti-GPR120/FFAR4 (extracellular) Antibody , preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse colon (lanes 9 and 11) and human colorectal adenocarcinoma (Colo 205) cell line (lanes 10 and 12) lysates: - 9,10. Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:200).11,12. Anti-GPR120/FFAR4 (extracellular) Antibody , preincubated with GPR120/FFAR4 (extracellular) Blocking Peptide (#BLP-FR014).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of FFAR4 in rat hypothalamus. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (1:1200), followed by biotinylated donkey Anti-rabbit and streptavidin-Cy3. A. FFAR4 immunoreactivity (red) appears in axons beaded with varicosities (arrows). B. Pre-incubation of the Antibody with GPR120/FFAR4 (extracellular) Blocking Peptide (BLP-FR014), suppressed staining. Cell nuclei are stained with DAPI (blue). VMH = ventromedial hypothalamus, 3V = 3rd ventricle.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of FFAR4 inlive intact human THP-1 monocytic leukemia cells: - (black line) cells. (red) Cells + goat Anti-rabbit-FITC. (green) Cells + Anti-GPR120/FFAR4 (extracellular) Antibody (#AFR-014), (2.5 μg/0.5x106 cells) + goat Anti-rabbit-FITC.