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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [17]
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- Western blot [2]
- Immunohistochemistry [2]
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- Product number
- NBP1-00858 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-00858, RRID:AB_1503311
- Product name
- Rabbit Polyclonal FFAR4/GPR120 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression and Secretion of an Atrial Natriuretic Peptide in Beige-Like 3T3-L1 Adipocytes.
Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus.
Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function.
GPR120 is not required for ω-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells.
Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120).
Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance.
ω-3 Polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells.
Insulinotropic effects of GPR120 agonists are altered in obese diabetic and obese non-diabetic states.
Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal β-arrestin phosphosensor in Raw 264.7 macrophages.
RA and ω-3 PUFA co-treatment activates autophagy in cancer cells.
A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure.
Branched Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) Protect against Colitis by Regulating Gut Innate and Adaptive Immune Responses.
A novel anti-inflammatory role of GPR120 in intestinal epithelial cells.
Omega-3 fatty acids induce Ca(2+) mobilization responses in human colon epithelial cell lines endogenously expressing FFA4.
Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer.
The fish oil ingredient, docosahexaenoic acid, activates cytosolic phospholipase A₂ via GPR120 receptor to produce prostaglandin E₂ and plays an anti-inflammatory role in macrophages.
Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary N-3 fatty acids.
Bae IS, Kim SH
International journal of molecular sciences 2019 Dec 5;20(24)
International journal of molecular sciences 2019 Dec 5;20(24)
Reduced expression of stearoyl-CoA desaturase-1, but not free fatty acid receptor 2 or 4 in subcutaneous adipose tissue of patients with newly diagnosed type 2 diabetes mellitus.
Bódis K, Kahl S, Simon MC, Zhou Z, Sell H, Knebel B, Tura A, Strassburger K, Burkart V, Müssig K, Markgraf D, Al-Hasani H, Szendroedi J, Roden M, GDS Study Group
Nutrition & diabetes 2018 Sep 7;8(1):49
Nutrition & diabetes 2018 Sep 7;8(1):49
Expression of the candidate fat taste receptors in human fungiform papillae and the association with fat taste function.
Liu D, Costanzo A, Evans MDM, Archer NS, Nowson C, Duesing K, Keast R
The British journal of nutrition 2018 Jul;120(1):64-73
The British journal of nutrition 2018 Jul;120(1):64-73
GPR120 is not required for ω-3 PUFAs-induced cell growth inhibition and apoptosis in breast cancer cells.
Zhu S, Jiang X, Jiang S, Lin G, Gong J, Chen W, He Z, Chen YQ
Cell biology international 2018 Feb;42(2):180-186
Cell biology international 2018 Feb;42(2):180-186
Omega-3 polyunsaturated fatty acids protect human hepatoma cells from developing steatosis through FFA4 (GPR120).
Kang S, Huang J, Lee BK, Jung YS, Im E, Koh JM, Im DS
Biochimica et biophysica acta. Molecular and cell biology of lipids 2018 Feb;1863(2):105-116
Biochimica et biophysica acta. Molecular and cell biology of lipids 2018 Feb;1863(2):105-116
Fatty acid 16:4(n-3) stimulates a GPR120-induced signaling cascade in splenic macrophages to promote chemotherapy resistance.
Houthuijzen JM, Oosterom I, Hudson BD, Hirasawa A, Daenen LGM, McLean CM, Hansen SVF, van Jaarsveld MTM, Peeper DS, Jafari Sadatmand S, Roodhart JML, van de Lest CHA, Ulven T, Ishihara K, Milligan G, Voest EE
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 May;31(5):2195-2209
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2017 May;31(5):2195-2209
ω-3 Polyunsaturated fatty acids accelerate airway repair by activating FFA4 in club cells.
Lee KP, Park SJ, Kang S, Koh JM, Sato K, Chung HY, Okajima F, Im DS
American journal of physiology. Lung cellular and molecular physiology 2017 Jun 1;312(6):L835-L844
American journal of physiology. Lung cellular and molecular physiology 2017 Jun 1;312(6):L835-L844
Insulinotropic effects of GPR120 agonists are altered in obese diabetic and obese non-diabetic states.
Zhang D, So WY, Wang Y, Wu SY, Cheng Q, Leung PS
Clinical science (London, England : 1979) 2017 Feb 1;131(3):247-260
Clinical science (London, England : 1979) 2017 Feb 1;131(3):247-260
Free-fatty acid receptor-4 (FFA4) modulates ROS generation and COX-2 expression via the C-terminal β-arrestin phosphosensor in Raw 264.7 macrophages.
Cheshmehkani A, Senatorov IS, Dhuguru J, Ghoneim O, Moniri NH
Biochemical pharmacology 2017 Dec 15;146:139-150
Biochemical pharmacology 2017 Dec 15;146:139-150
RA and ω-3 PUFA co-treatment activates autophagy in cancer cells.
Zhu S, Lin G, Song C, Wu Y, Feng N, Chen W, He Z, Chen YQ
Oncotarget 2017 Dec 12;8(65):109135-109150
Oncotarget 2017 Dec 12;8(65):109135-109150
A Single-Cell Transcriptomic Map of the Human and Mouse Pancreas Reveals Inter- and Intra-cell Population Structure.
Baron M, Veres A, Wolock SL, Faust AL, Gaujoux R, Vetere A, Ryu JH, Wagner BK, Shen-Orr SS, Klein AM, Melton DA, Yanai I
Cell systems 2016 Oct 26;3(4):346-360.e4
Cell systems 2016 Oct 26;3(4):346-360.e4
Branched Fatty Acid Esters of Hydroxy Fatty Acids (FAHFAs) Protect against Colitis by Regulating Gut Innate and Adaptive Immune Responses.
Lee J, Moraes-Vieira PM, Castoldi A, Aryal P, Yee EU, Vickers C, Parnas O, Donaldson CJ, Saghatelian A, Kahn BB
The Journal of biological chemistry 2016 Oct 14;291(42):22207-22217
The Journal of biological chemistry 2016 Oct 14;291(42):22207-22217
A novel anti-inflammatory role of GPR120 in intestinal epithelial cells.
Anbazhagan AN, Priyamvada S, Gujral T, Bhattacharyya S, Alrefai WA, Dudeja PK, Borthakur A
American journal of physiology. Cell physiology 2016 Apr 1;310(7):C612-21
American journal of physiology. Cell physiology 2016 Apr 1;310(7):C612-21
Omega-3 fatty acids induce Ca(2+) mobilization responses in human colon epithelial cell lines endogenously expressing FFA4.
Kim JM, Lee KP, Park SJ, Kang S, Huang J, Lee JM, Sato K, Chung HY, Okajima F, Im DS
Acta pharmacologica Sinica 2015 Jul;36(7):813-20
Acta pharmacologica Sinica 2015 Jul;36(7):813-20
Omega-3 fatty acids reduce obesity-induced tumor progression independent of GPR120 in a mouse model of postmenopausal breast cancer.
Chung H, Lee YS, Mayoral R, Oh DY, Siu JT, Webster NJ, Sears DD, Olefsky JM, Ellies LG
Oncogene 2015 Jul;34(27):3504-13
Oncogene 2015 Jul;34(27):3504-13
The fish oil ingredient, docosahexaenoic acid, activates cytosolic phospholipase A₂ via GPR120 receptor to produce prostaglandin E₂ and plays an anti-inflammatory role in macrophages.
Liu Y, Chen LY, Sokolowska M, Eberlein M, Alsaaty S, Martinez-Anton A, Logun C, Qi HY, Shelhamer JH
Immunology 2014 Sep;143(1):81-95
Immunology 2014 Sep;143(1):81-95
Identification of a molecular signature underlying inhibition of mammary carcinoma growth by dietary N-3 fatty acids.
Jiang W, Zhu Z, McGinley JN, El Bayoumy K, Manni A, Thompson HJ
Cancer research 2012 Aug 1;72(15):3795-806
Cancer research 2012 Aug 1;72(15):3795-806
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - Analysis of GPR120 (V259) antibody in extracts from LOVO cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FFAR4/GPR120 Antibody [NBP1-00858] - GPR120 expression in mouse spleen cells (1), bone marrow (2), bone marrow derived DC (3) and macrophages (4). This image was submitted through a verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein ), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FFAR4/GPR120 Antibody [NBP1-00858] - IHC analysis of formalin fixed paraffin embedded tissue section of mouse intestine using FFAR4/GPR120 antibody at 1:200 dilution. The intestinal epithelial cells showed an expected cytoplasmic staining (immunogen of NBP1-00858 corresponds to the cytoplasmic domain of GPR120 protein), while some cells especially the goblet cells population depicted nuclear positivity also. The observed nuclear signal may be justified on the fact that - some GPCRs are known for nuclear translocation upon binding to extracellular or endogenous/non-secreted ligands, wherein they impact the trancriptional regulation via GPCR-heterotrimeric G-protein-effector complexes.
