LS-B6446

antibody from LSBio

Targeting: DAP3 bMRP-10, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29

ELISA

Immunocytochemistry

Immunohistochemistry

Antibody data

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Validation data

Product number: LS-B6446 - Provider product page
Provider: LSBio
Product name: IHC-plus™ DAP3 Antibody (aa387-398) LS-B6446
Antibody type: Polyclonal
Description: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Reactivity: Human, Simian
Host: Goat
Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

DAP3 protein structure - LS-B6446 shown in red.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: DAP3 antibody staining (3 ug/ml) of HeLa lysate (RIPA buffer, 30g total protein per lane). Primary incubated for 1 hour. Detected by Western blot of chemiluminescence.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Death Associated Protein 3 antibody (0.3µg/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Death Associated Protein 3 antibody (1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was determined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Death Associated Protein 3 antibody staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Supportive validation

Submitted by: LSBio (provider)
Enhanced method: Genetic validation
Main image
Experimental details: Goat Anti-DAP3 Antibody (0.3µg/ml) staining of HeLa (A) and HepG2 (B) cell lysate (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescencence.