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LearnOAEB01341
antibody from Aviva Systems Biology
Targeting: DAP3
bMRP-10, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- OAEB01341 - Provider product page
- Provider
- Aviva Systems Biology
- Proper citation
- Aviva Systems Biology Cat#OAEB01341, RRID:AB_10895373
- Product name
- DAP3 Antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Bovine
- Host
- Goat
- Vial size
- 0.1mg
- Storage
- Aliquot and store at -20°C. Minimize freezing and thawing.
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Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- HEK293 overexpressing DAP3 (RC223182) and probed with OAEB01341 (mock transfection in first lane), tested by Origene.
- Sample type
- DAP3 (RC223182)
- Primary Ab dilution
- 3 µg/mL
- Protocol
- Protocol
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- OAEB01341 staining (3µg/ml) of HeLa lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Sample type
- Hela cell lysate
- Primary Ab dilution
- 1.0 µg/mL
- Protocol
- Protocol
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- OAEB01341 (0.5µg/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.
