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LearnARG63209
antibody from Arigo
Targeting: DAP3
bMRP-10, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- ARG63209 - Provider product page
- Provider
- Arigo
- Product name
- anti-DAP3 antibody
- Antibody type
- Polyclonal
- Antigen
- NPSLLERHCAYL
- Description
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35 µg protein in RIPA buffer) Level of knock-down relative to Actin expression level was determined by RT-PCR. stained with ARG63209 anti-DAP3 antibody at 1 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: HeLa lysate (RIPA buffer, 30µg total protein per lane) stained with ARG63209 anti-DAP3 antibody at 3 µg/ml dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western Blot: 1). Mock transfection; 2) DAP3 (RC223182) expressing plasmid transfected HEK293 cell lysate standed with ARG63209 anti-DAP3 antibody
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunofluorescence: guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson stained with ARG63209 anti-DAP3 antibod (0.5ug/ml). Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.
