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LearnAP16015PU-N
antibody from Acris Antibodies GmbH
Targeting: DAP3
bMRP-10, DAP-3, DKFZp686G12159, MGC126058, MGC126059, MRP-S29, MRPS29
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
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- Product number
- AP16015PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP16015PU-N, RRID:AB_1927986
- Product name
- anti DAP3 / MRPS29 (C-term)
- Antibody type
- Polyclonal
- Antigen
- Peptide from C-Terminus of the protein sequence according to NP_387506.1; NP_004623.1
- Reactivity
- Human
- Host
- Goat
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16015PU-N staining (3 µg/ml) of HeLa lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- HEK293 overexpressing DAP3 and probed with AP16015PU-N (mock transfection in first lane), tested by Origene.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16015PU-N (1µg/ml) staining of HeLa lysate (control in left lane and after si-RNA-mediated DAP3 knock-down expresson in right lane) (35µg protein in RIPA buffer). Level of knock-down relative to Actin expression level was detemined by RT-PCR. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- AP16015PU-N (0.5 mg/ml) staining of guanidinium thiocyanate-treated HeLa before (left) and after (right) si-RNA-mediated DAP3 knock-down expresson. Primary incubation 1h at ambient temp. Detection by DyLight 488. Nuclear DAPI stain.
