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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [2]
- Other assay [1]
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- Product number
- MA1-19590 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD80 Monoclonal Antibody (MEM-233), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- MEM-233
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Development of a skin- and neuro-attenuated live vaccine for varicella.
Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Wang W, Pan D, Fu W, Ye X, Han J, Yang L, Jia J, Liu J, Zhu R, Zhang Y, Liu C, Ye J, Selariu A, Que Y, Zhao Q, Wu T, Li Y, Zhang J, Cheng T, Zhu H, Xia N
Nature communications 2022 Feb 11;13(1):824
Nature communications 2022 Feb 11;13(1):824
Major histocompatibility complex class II (DR) antigen and costimulatory molecules on in vitro and in vivo activated human polymorphonuclear neutrophils.
Sandilands GP, McCrae J, Hill K, Perry M, Baxter D
Immunology 2006 Dec;119(4):562-71
Immunology 2006 Dec;119(4):562-71
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Zingoni A, Sornasse T, Cocks BG, Tanaka Y, Santoni A, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Cross-talk between activated human NK cells and CD4+ T cells via OX40-OX40 ligand interactions.
Zingoni A, Sornasse T, Cocks BG, Tanaka Y, Santoni A, Lanier LL
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 15;173(6):3716-24
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
CD80 and CD86 C domains play an important role in receptor binding and co-stimulatory properties.
Vasu C, Wang A, Gorla SR, Kaithamana S, Prabhakar BS, Holterman MJ
International immunology 2003 Feb;15(2):167-75
International immunology 2003 Feb;15(2):167-75
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of CD80 using a monoclonal antibody (Product # MA1-19590).
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis (surface staining) of CD80 transfected P815 cells using anti-human CD80 (MEM-233) FITC Monoclonal antibody (Product # MA1-19590) (red, concentration in sample 3 µg/mL) with blank sample (grey).
- Conjugate
- Green dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 v7D induces functional activation of human DCs in vitro. Human iDCs were generated from CD14 + monocytes purified from PBMCs, and were treated with rOka, v7D, vOka (MOI = 0.01) or mock-infected MRC-5 cell lysate as a control. a , b Representative flow cytometry data and summary of VZV-gE expression on iDCs at 3 dpi. rOka-infected iDCs were stained with an isotype antibody (iso) as a control. Numbers on the right side of each graph in ( a ) represent the percent of VZV-gE-positive cells. c Flow cytometry analysis of expression of the cell-surface costimulatory markers CD40, CD80, CD83 and CD86 on DCs at 3 dpi. Representative flow cytometry data are shown in Supplementary Fig. 5 . d Analysis of percent lysis of DCs compared to that of MRC-5 cells after inoculation with v7D and vOka (MOI = 0.01) over five days. e Cytokine/chemokine analysis of DC culture supernatants. f Analysis of cell proliferation rates of the purified autologous CD4 + and CD8 + T cells after co-culture with antigen-pulsed DCs using the BrdU ELISA assay. g , h Representative flow cytometry data and summary data of CFSE dilution among CD4 + and CD8 + T cells after 5-day co-culture with antigen-pulsed DCs. i Analysis of IFN-gamma production in CD4 + and CD8 + T cells after 5 days of co-culture with antigen-pulsed DCs using an ELISPOT assay. The results are represented as averages +- the SD ( n = 3 per group). Asterisks denote a significant difference ( P < 0.05) compared to the mock controls as determine
- Conjugate
- Green dye
