Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [39]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [4]
- Immunohistochemistry [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NB100-1604 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1604, RRID:AB_2282642
- Product name
- Chicken Polyclonal Nestin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.25 ml
- Concentration
- 0.3 mg/ml
- Storage
- Store at 4C in the dark.
Submitted references Tanycytic TSPO inhibition induces lipophagy to regulate lipid metabolism and improve energy balance.
BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation.
Early Seizure Activity Accelerates Depletion of Hippocampal Neural Stem Cells and Impairs Spatial Discrimination in an Alzheimer's Disease Model.
Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon.
Optimized Clump Culture Methods for Adult Human Multipotent Neural Cells.
The Thrombin Receptor Restricts Subventricular Zone Neural Stem Cell Expansion and Differentiation.
Somatosensory innervation of the oral mucosa of adult and aging mice.
Stress-induced unfolded protein response contributes to Zika virus-associated microcephaly.
Genetic dissection of the neuro-glio-vascular machinery in the adult brain.
Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen.
Spatiotemporal presentation of exogenous SDF-1 with PLGA nanoparticles modulates SDF-1/CXCR4 signaling axis in the rodent cortex.
CXCR7 Participates in CXCL12-mediated Cell Cycle and Proliferation Regulation in Mouse Neural Progenitor Cells.
CXCR7 Participates in CXCL12-mediated Cell Cycle and Proliferation Regulation in Mouse Neural Progenitor Cells.
Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
Loss of endogenous thymosin β(4) accelerates glomerular disease.
Neural stem/progenitor cells differentiate into oligodendrocytes, reduce inflammation, and ameliorate learning deficits after transplantation in a mouse model of traumatic brain injury.
Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brain.
IL-6 Trans-Signaling Drives Murine Crescentic GN.
CXCR4 increases in-vivo glioma perivascular invasion, and reduces radiation induced apoptosis: A genetic knockdown study.
Leaves of Persimmon (Diospyros kaki Thunb.) Ameliorate N-Methyl-N-nitrosourea (MNU)-Induced Retinal Degeneration in Mice.
Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury.
Neural stem cell transplantation in an animal model of traumatic brain injury.
Additive or synergistic effects of aluminum on the reduction of neural stem cells, cell proliferation, and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice.
Adult bone marrow neural crest stem cells and mesenchymal stem cells are not able to replace lost neurons in acute MPTP-lesioned mice.
Evolutionary etiology of high-grade astrocytomas.
MHC class I protein is expressed by neurons and neural progenitors in mid-gestation mouse brain.
Effects of treadmill exercise on neural stem cells, cell proliferation, and neuroblast differentiation in the subgranular zone of the dentate gyrus in cyclooxygenase-2 knockout mice.
GFAP isoforms in adult mouse brain with a focus on neurogenic astrocytes and reactive astrogliosis in mouse models of Alzheimer disease.
GFAPδ expression in glia of the developmental and adolescent mouse brain.
In vivo tumorigenesis was observed after injection of in vitro expanded neural crest stem cells isolated from adult bone marrow.
Diazepam binding inhibitor promotes progenitor proliferation in the postnatal SVZ by reducing GABA signaling.
Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Interferon β-1b directly modulates human neural stem/progenitor cell fate.
Nestin expression associates with poor prognosis and triple negative phenotype in locally advanced (T4) breast cancer.
Reprogrammed mouse astrocytes retain a "memory" of tissue origin and possess more tendencies for neuronal differentiation than reprogrammed mouse embryonic fibroblasts.
ProSAPiP2, a novel postsynaptic density protein that interacts with ProSAP2/Shank3.
GFAP-GFP neural progenitors are antigenically homogeneous and anchored in their enclosed mosaic niche.
MYCN promotes the expansion of Phox2B-positive neuronal progenitors to drive neuroblastoma development.
Nestin expression defines both glial and neuronal progenitors in postnatal sympathetic ganglia.
Kim S, Kim N, Park S, Jeon Y, Lee J, Yoo SJ, Lee JW, Moon C, Yu SW, Kim EK
Autophagy 2020 Jul;16(7):1200-1220
Autophagy 2020 Jul;16(7):1200-1220
BMP4 and Neuregulin regulate the direction of mouse neural crest cell differentiation.
Zhu S, Liu W, Ding HF, Cui H, Yang L
Experimental and therapeutic medicine 2019 May;17(5):3883-3890
Experimental and therapeutic medicine 2019 May;17(5):3883-3890
Early Seizure Activity Accelerates Depletion of Hippocampal Neural Stem Cells and Impairs Spatial Discrimination in an Alzheimer's Disease Model.
Fu CH, Iascone DM, Petrof I, Hazra A, Zhang X, Pyfer MS, Tosi U, Corbett BF, Cai J, Lee J, Park J, Iacovitti L, Scharfman HE, Enikolopov G, Chin J
Cell reports 2019 Jun 25;27(13):3741-3751.e4
Cell reports 2019 Jun 25;27(13):3741-3751.e4
Zika virus infection at mid-gestation results in fetal cerebral cortical injury and fetal death in the olive baboon.
Gurung S, Reuter N, Preno A, Dubaut J, Nadeau H, Hyatt K, Singleton K, Martin A, Parks WT, Papin JF, Myers DA
PLoS pathogens 2019 Jan;15(1):e1007507
PLoS pathogens 2019 Jan;15(1):e1007507
Optimized Clump Culture Methods for Adult Human Multipotent Neural Cells.
Yeon JY, Hwang JY, Lee HW, Pyeon HJ, Won JS, Noh YJ, Nam H, Joo KM
International journal of molecular sciences 2018 Oct 29;19(11)
International journal of molecular sciences 2018 Oct 29;19(11)
The Thrombin Receptor Restricts Subventricular Zone Neural Stem Cell Expansion and Differentiation.
Choi CI, Yoon H, Drucker KL, Langley MR, Kleppe L, Scarisbrick IA
Scientific reports 2018 Jun 19;8(1):9360
Scientific reports 2018 Jun 19;8(1):9360
Somatosensory innervation of the oral mucosa of adult and aging mice.
Moayedi Y, Duenas-Bianchi LF, Lumpkin EA
Scientific reports 2018 Jul 2;8(1):9975
Scientific reports 2018 Jul 2;8(1):9975
Stress-induced unfolded protein response contributes to Zika virus-associated microcephaly.
Gladwyn-Ng I, Cordón-Barris L, Alfano C, Creppe C, Couderc T, Morelli G, Thelen N, America M, Bessières B, Encha-Razavi F, Bonnière M, Suzuki IK, Flamand M, Vanderhaeghen P, Thiry M, Lecuit M, Nguyen L
Nature neuroscience 2018 Jan;21(1):63-71
Nature neuroscience 2018 Jan;21(1):63-71
Genetic dissection of the neuro-glio-vascular machinery in the adult brain.
Kirschen GW, Kéry R, Liu H, Ahamad A, Chen L, Akmentin W, Kumar R, Levine J, Xiong Q, Ge S
Molecular brain 2018 Jan 15;11(1):2
Molecular brain 2018 Jan 15;11(1):2
Neonatal Subventricular Zone Neural Stem Cells Release Extracellular Vesicles that Act as a Microglial Morphogen.
Morton MC, Neckles VN, Seluzicki CM, Holmberg JC, Feliciano DM
Cell reports 2018 Apr 3;23(1):78-89
Cell reports 2018 Apr 3;23(1):78-89
Spatiotemporal presentation of exogenous SDF-1 with PLGA nanoparticles modulates SDF-1/CXCR4 signaling axis in the rodent cortex.
Dutta D, Hickey K, Salifu M, Fauer C, Willingham C, Stabenfeldt SE
Biomaterials science 2017 Jul 25;5(8):1640-1651
Biomaterials science 2017 Jul 25;5(8):1640-1651
CXCR7 Participates in CXCL12-mediated Cell Cycle and Proliferation Regulation in Mouse Neural Progenitor Cells.
Wang Y, Xu P, Qiu L, Zhang M, Huang Y, Zheng JC
Current molecular medicine 2016;16(8):738-746
Current molecular medicine 2016;16(8):738-746
CXCR7 Participates in CXCL12-mediated Cell Cycle and Proliferation Regulation in Mouse Neural Progenitor Cells.
Wang Y, Xu P, Qiu L, Zhang M, Huang Y, Zheng JC
Current molecular medicine 2016;16(8):738-746
Current molecular medicine 2016;16(8):738-746
Dopaminergic neurons differentiating from LRRK2 G2019S induced pluripotent stem cells show early neuritic branching defects.
Borgs L, Peyre E, Alix P, Hanon K, Grobarczyk B, Godin JD, Purnelle A, Krusy N, Maquet P, Lefebvre P, Seutin V, Malgrange B, Nguyen L
Scientific reports 2016 Sep 19;6:33377
Scientific reports 2016 Sep 19;6:33377
Loss of endogenous thymosin β(4) accelerates glomerular disease.
Vasilopoulou E, Kolatsi-Joannou M, Lindenmeyer MT, White KE, Robson MG, Cohen CD, Sebire NJ, Riley PR, Winyard PJ, Long DA
Kidney international 2016 Nov;90(5):1056-1070
Kidney international 2016 Nov;90(5):1056-1070
Neural stem/progenitor cells differentiate into oligodendrocytes, reduce inflammation, and ameliorate learning deficits after transplantation in a mouse model of traumatic brain injury.
Koutsoudaki PN, Papastefanaki F, Stamatakis A, Kouroupi G, Xingi E, Stylianopoulou F, Matsas R
Glia 2016 May;64(5):763-79
Glia 2016 May;64(5):763-79
Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brain.
Fantetti KN, Gray EL, Ganesan P, Kulkarni A, O'Donnell LA
Journal of neuroinflammation 2016 May 13;13(1):107
Journal of neuroinflammation 2016 May 13;13(1):107
IL-6 Trans-Signaling Drives Murine Crescentic GN.
Braun GS, Nagayama Y, Maruta Y, Heymann F, van Roeyen CR, Klinkhammer BM, Boor P, Villa L, Salant DJ, Raffetseder U, Rose-John S, Ostendorf T, Floege J
Journal of the American Society of Nephrology : JASN 2016 Jan;27(1):132-42
Journal of the American Society of Nephrology : JASN 2016 Jan;27(1):132-42
CXCR4 increases in-vivo glioma perivascular invasion, and reduces radiation induced apoptosis: A genetic knockdown study.
Yadav VN, Zamler D, Baker GJ, Kadiyala P, Erdreich-Epstein A, DeCarvalho AC, Mikkelsen T, Castro MG, Lowenstein PR
Oncotarget 2016 Dec 13;7(50):83701-83719
Oncotarget 2016 Dec 13;7(50):83701-83719
Leaves of Persimmon (Diospyros kaki Thunb.) Ameliorate N-Methyl-N-nitrosourea (MNU)-Induced Retinal Degeneration in Mice.
Kim KA, Kang SW, Ahn HR, Song Y, Yang SJ, Jung SH
Journal of agricultural and food chemistry 2015 Sep 9;63(35):7750-9
Journal of agricultural and food chemistry 2015 Sep 9;63(35):7750-9
Adult bone marrow mesenchymal and neural crest stem cells are chemoattractive and accelerate motor recovery in a mouse model of spinal cord injury.
Neirinckx V, Agirman G, Coste C, Marquet A, Dion V, Rogister B, Franzen R, Wislet S
Stem cell research & therapy 2015 Nov 4;6:211
Stem cell research & therapy 2015 Nov 4;6:211
Neural stem cell transplantation in an animal model of traumatic brain injury.
Thomaidou D
Methods in molecular biology (Clifton, N.J.) 2014;1210:9-21
Methods in molecular biology (Clifton, N.J.) 2014;1210:9-21
Additive or synergistic effects of aluminum on the reduction of neural stem cells, cell proliferation, and neuroblast differentiation in the dentate gyrus of high-fat diet-fed mice.
Nam SM, Kim JW, Yoo DY, Kim W, Jung HY, Hwang IK, Seong JK, Yoon YS
Biological trace element research 2014 Jan;157(1):51-9
Biological trace element research 2014 Jan;157(1):51-9
Adult bone marrow neural crest stem cells and mesenchymal stem cells are not able to replace lost neurons in acute MPTP-lesioned mice.
Neirinckx V, Marquet A, Coste C, Rogister B, Wislet-Gendebien S
PloS one 2013;8(5):e64723
PloS one 2013;8(5):e64723
Evolutionary etiology of high-grade astrocytomas.
Song Y, Zhang Q, Kutlu B, Difilippantonio S, Bash R, Gilbert D, Yin C, O'Sullivan TN, Yang C, Kozlov S, Bullitt E, McCarthy KD, Kafri T, Louis DN, Miller CR, Hood L, Van Dyke T
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 29;110(44):17933-8
Proceedings of the National Academy of Sciences of the United States of America 2013 Oct 29;110(44):17933-8
MHC class I protein is expressed by neurons and neural progenitors in mid-gestation mouse brain.
Chacon MA, Boulanger LM
Molecular and cellular neurosciences 2013 Jan;52:117-27
Molecular and cellular neurosciences 2013 Jan;52:117-27
Effects of treadmill exercise on neural stem cells, cell proliferation, and neuroblast differentiation in the subgranular zone of the dentate gyrus in cyclooxygenase-2 knockout mice.
Nam SM, Kim JW, Yoo DY, Choi JH, Kim W, Jung HY, Won MH, Hwang IK, Seong JK, Yoon YS
Neurochemical research 2013 Dec;38(12):2559-69
Neurochemical research 2013 Dec;38(12):2559-69
GFAP isoforms in adult mouse brain with a focus on neurogenic astrocytes and reactive astrogliosis in mouse models of Alzheimer disease.
Kamphuis W, Mamber C, Moeton M, Kooijman L, Sluijs JA, Jansen AH, Verveer M, de Groot LR, Smith VD, Rangarajan S, Rodríguez JJ, Orre M, Hol EM
PloS one 2012;7(8):e42823
PloS one 2012;7(8):e42823
GFAPδ expression in glia of the developmental and adolescent mouse brain.
Mamber C, Kamphuis W, Haring NL, Peprah N, Middeldorp J, Hol EM
PloS one 2012;7(12):e52659
PloS one 2012;7(12):e52659
In vivo tumorigenesis was observed after injection of in vitro expanded neural crest stem cells isolated from adult bone marrow.
Wislet-Gendebien S, Poulet C, Neirinckx V, Hennuy B, Swingland JT, Laudet E, Sommer L, Shakova O, Bours V, Rogister B
PloS one 2012;7(10):e46425
PloS one 2012;7(10):e46425
Diazepam binding inhibitor promotes progenitor proliferation in the postnatal SVZ by reducing GABA signaling.
Alfonso J, Le Magueresse C, Zuccotti A, Khodosevich K, Monyer H
Cell stem cell 2012 Jan 6;10(1):76-87
Cell stem cell 2012 Jan 6;10(1):76-87
Mesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Wislet-Gendebien S, Laudet E, Neirinckx V, Alix P, Leprince P, Glejzer A, Poulet C, Hennuy B, Sommer L, Shakhova O, Rogister B
Cellular and molecular life sciences : CMLS 2012 Aug;69(15):2593-608
Cellular and molecular life sciences : CMLS 2012 Aug;69(15):2593-608
Interferon β-1b directly modulates human neural stem/progenitor cell fate.
Arscott WT, Soltys J, Knight J, Mao-Draayer Y
Brain research 2011 Sep 21;1413:1-8
Brain research 2011 Sep 21;1413:1-8
Nestin expression associates with poor prognosis and triple negative phenotype in locally advanced (T4) breast cancer.
Piras F, Ionta MT, Lai S, Perra MT, Atzori F, Minerba L, Pusceddu V, Maxia C, Murtas D, Demurtas P, Massidda B, Sirigu P
European journal of histochemistry : EJH 2011 Nov 14;55(4):e39
European journal of histochemistry : EJH 2011 Nov 14;55(4):e39
Reprogrammed mouse astrocytes retain a "memory" of tissue origin and possess more tendencies for neuronal differentiation than reprogrammed mouse embryonic fibroblasts.
Tian C, Wang Y, Sun L, Ma K, Zheng JC
Protein & cell 2011 Feb;2(2):128-40
Protein & cell 2011 Feb;2(2):128-40
ProSAPiP2, a novel postsynaptic density protein that interacts with ProSAP2/Shank3.
Liebau S, Proepper C, Schmidt T, Schoen M, Bockmann J, Boeckers TM
Biochemical and biophysical research communications 2009 Jul 31;385(3):460-5
Biochemical and biophysical research communications 2009 Jul 31;385(3):460-5
GFAP-GFP neural progenitors are antigenically homogeneous and anchored in their enclosed mosaic niche.
Platel JC, Gordon V, Heintz T, Bordey A
Glia 2009 Jan 1;57(1):66-78
Glia 2009 Jan 1;57(1):66-78
MYCN promotes the expansion of Phox2B-positive neuronal progenitors to drive neuroblastoma development.
Alam G, Cui H, Shi H, Yang L, Ding J, Mao L, Maltese WA, Ding HF
The American journal of pathology 2009 Aug;175(2):856-66
The American journal of pathology 2009 Aug;175(2):856-66
Nestin expression defines both glial and neuronal progenitors in postnatal sympathetic ganglia.
Shi H, Cui H, Alam G, Gunning WT, Nestor A, Giovannucci D, Zhang M, Ding HF
The Journal of comparative neurology 2008 Jun 20;508(6):867-78
The Journal of comparative neurology 2008 Jun 20;508(6):867-78
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody [NB100-1604] - 10ug of homogenate from e18 mouse brains, 7.5% SDS-PAGE. Detection performed with an HRP-conjugated Goat Anti-Chicken IgY antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: Nestin Antibody [NB100-1604] - Analysis of Nestin in frozen mouse tissue using anti-Nestin antibody. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Nestin Antibody [NB100-1604] - A tissue section through an e 13 mouse brain showing Nestin (green staining) in neural stem cells of the ventricular zone of the CNS. DAPI (blue staining) allows visualization of nuclei. The fixative was 2% paraformaldehyde in PBS, and the tissue was emersed in this fixative for 30 minutes at room temperature.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NB100-1604] - Photomicrograph of a paraffin-embedded tissue section through an adult dentate gyrus of the hippocampal formation from a paraformaldehyde-fixed (4%) mouse brain. RED shows nestin immunoreactivity as visualized with a Texas Red goat anti-chicken IgY antibody. Green is staining of the granule cells; BLUE is DAPI nuclear staining.
