MA1-5840

antibody from Invitrogen Antibodies

Targeting: NES FLJ21841

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-5840

Provider

Invitrogen Antibodies

Product name

Nestin Monoclonal Antibody (2C1.3A11)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-5840 detects Nestin in human samples, and does not cross-react with rodent.

Antibody clone number

2C1.3A11

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Nestin Monoclonal Antibody (2C1.3A11) (Product # MA1-5840) and a 200 kDa band corresponding to Nestin was observed in NSC and the expression was increased in NSC differentiated to neurons. Whole cell extracts (30 µg lysate) of iPSC (Lane 1), NSC (Lane 2), NSC differentiated to neurons (Lane 3) and Jurkat (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Nestin in formalin-fixed paraffin embedded prostate nerve tissue using monoclonal antibody (Product # MA1-5840).