IC1259P

antibody from Novus Biologicals

Targeting: NES FLJ21841

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC1259P

Provider

Novus Biologicals

Product name

Mouse Monoclonal Nestin Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Nestin.

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.

References

Submitted references

Evaluation of apoptosis and angiogenesis in ectopic and eutopic stromal cells of patients with endometriosis compared to non-endometriotic controls.

Delbandi AA, Mahmoudi M, Shervin A, Heidari S, Kolahdouz-Mohammadi R, Zarnani AH
BMC women's health 2020 Jan 6;20(1):3

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Bioengineering Human Neurological Constructs Using Decellularized Meningeal Scaffolds for Application in Spinal Cord Injury.

Vishwakarma SK, Bardia A, Lakkireddy C, Paspala SAB, Khan AA
Frontiers in bioengineering and biotechnology 2018;6:150

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The spleen of patients with myelofibrosis harbors defective mesenchymal stromal cells.

Avanzini MA, Abbonante V, Catarsi P, Dambruoso I, Mantelli M, Poletto V, Lenta E, Guglielmelli P, Croce S, Cobianchi L, Jemos B, Campanelli R, Bonetti E, Di Buduo CA, Salmoiraghi S, Villani L, Massa M, Boni M, Zappatore R, Iurlo A, Rambaldi A, Vannucchi AM, Bernasconi P, Balduini A, Barosi G, Rosti V, AGIMM Investigators.
American journal of hematology 2018 May;93(5):615-622

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HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.

Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092

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Phenotypic diversity of patient-derived melanoma populations in stem cell medium.

Sztiller-Sikorska M, Hartman ML, Talar B, Jakubowska J, Zalesna I, Czyz M
Laboratory investigation; a journal of technical methods and pathology 2015 Jun;95(6):672-83

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Expression of the stem cell markers nestin and CD133 on circulating melanoma cells.

Fusi A, Reichelt U, Busse A, Ochsenreither S, Rietz A, Maisel M, Keilholz U
The Journal of investigative dermatology 2011 Feb;131(2):487-94

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MDR1 expression identifies human melanoma stem cells.

Keshet GI, Goldstein I, Itzhaki O, Cesarkas K, Shenhav L, Yakirevitch A, Treves AJ, Schachter J, Amariglio N, Rechavi G
Biochemical and biophysical research communications 2008 Apr 18;368(4):930-6

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Nestin in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line either untreated (dark orange filled histogram) or treated with 5 μM Retinoic Acid for 60 days (light orange filled histogram) was stained with Mouse Anti-Human Nestin PE-conjugated Monoclonal Antibody (Catalog # IC1259P) or isotype control antibody (Catalog # IC002P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.