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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- TA324694 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Nestin Antibody (S1409)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Nestin Antibody (S1409)
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- NES
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- 0.375 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of Nestin Antibody (S1409) (Cat.#TA324694) in HepG2 cell line lysates (35ug/lane). NES (arrow) was detected using the purified Pab.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF image of SY5Y cells stained with Nestin (S1409) antibody. SY5Y cells were incubated with TA324694 Nestin (S1409) primary antibody (1:200, 2 h at RT). For secondary antibody, Alexa Fluor? 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) . Note the highly specific localization of the Nestin (S1409) immunosignal to the intermediate filaments.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using Nestin Antibody (S1409)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- FC
