Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP11887PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP11887PU-N, RRID:AB_1770012
- Product name
- anti Nestin
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 1389-1416 amino acids from Human Nestin (S1409)
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
Submitted references Identification of a novel putative pancreatic stem/progenitor cell marker DCAMKL-1 in normal mouse pancreas.
May R, Sureban SM, Lightfoot SA, Hoskins AB, Brackett DJ, Postier RG, Ramanujam R, Rao CV, Wyche JH, Anant S, Houchen CW
American journal of physiology. Gastrointestinal and liver physiology 2010 Aug;299(2):G303-10
American journal of physiology. Gastrointestinal and liver physiology 2010 Aug;299(2):G303-10
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis of Nestin Antibody (S1409) Cat.-No AP11887PU-N in HepG2 cell line lysates (35 µg/lane). NES (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Fluorescent confocal image of SY5Y cells stained with Nestin Antibody (S1409) Cat.-No AP11887PU-N. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with Nestin Antibody (S1409) Cat.-No AP11887PU-N (1/200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min). Note the highly specific localization of the Nestin (S1409) immunosignal to the intermediate filaments, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000132688).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with Nestin Antibody (S1409) Cat.-No AP11887PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HepG2 cells using Nestin Antibody (S1409) Cat.-No AP11887PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.