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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Flow cytometry [2]
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- Product number
- IC2736P - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse/Rat Nestin PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse and rat Nestin in Western blots.
- Reactivity
- Mouse, Rat
- Host
- Mouse
- Antigen sequence
EDM00749- Isotype
- IgG
- Antibody clone number
- 307501
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Cancer stem-like cells can be induced through dedifferentiation under hypoxic conditions in glioma, hepatoma and lung cancer.
The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals.
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile.
Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
Wang P, Wan WW, Xiong SL, Feng H, Wu N
Cell death discovery 2017;3:16105
Cell death discovery 2017;3:16105
The functional curcumin liposomes induce apoptosis in C6 glioblastoma cells and C6 glioblastoma stem cells in vitro and in animals.
Wang Y, Ying X, Xu H, Yan H, Li X, Tang H
International journal of nanomedicine 2017;12:1369-1384
International journal of nanomedicine 2017;12:1369-1384
HIF1α regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia.
Wang P, Lan C, Xiong S, Zhao X, Shan Y, Hu R, Wan W, Yu S, Liao B, Li G, Wang J, Zou D, Chen B, Feng H, Wu N
Oncotarget 2017 Apr 25;8(17):28074-28092
Oncotarget 2017 Apr 25;8(17):28074-28092
Thymic Mesenchymal Cells Have a Distinct Transcriptomic Profile.
Patenaude J, Perreault C
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4760-70
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4760-70
Activated CD8+ T lymphocytes inhibit neural stem/progenitor cell proliferation: role of interferon-gamma.
Hu S, Rotschafer JH, Lokensgard JR, Cheeran MC
PloS one 2014;9(8):e105219
PloS one 2014;9(8):e105219
Murine cytomegalovirus infection of neural stem cells alters neurogenesis in the developing brain.
Mutnal MB, Cheeran MC, Hu S, Lokensgard JR
PloS one 2011 Jan 13;6(1):e16211
PloS one 2011 Jan 13;6(1):e16211
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Nestin in Rat Cortical Stem Cells by Flow Cytometry. Rat cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE-conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Nestin in Mouse Cortical Stem Cells by Flow Cytometry. Mouse cortical stem cells were stained with Mouse Anti-Mouse/Rat Nestin PE-conjugated Monoclonal Antibody (Catalog # IC2736P, filled histogram) or isotype control antibody (Catalog # IC003P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
