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Western blot
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- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NBP1-02419 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-02419, RRID:AB_1521965
- Product name
- Rabbit Polyclonal Nestin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Amino acids 1484-1500 of human Nestin protein.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 mg
- Concentration
- 1 mg/ml
- Storage
- Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
Submitted references The RNA-Binding Protein RBM3 Promotes Neural Stem Cell (NSC) Proliferation Under Hypoxia.
Neural Stem Cells Derived Directly from Adipose Tissue.
Notch transactivates Rheb to maintain the multipotency of TSC-null cells.
Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell.
Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors.
Yan J, Goerne T, Zelmer A, Guzman R, Kapfhammer JP, Wellmann S, Zhu X
Frontiers in cell and developmental biology 2019;7:288
Frontiers in cell and developmental biology 2019;7:288
Neural Stem Cells Derived Directly from Adipose Tissue.
Petersen ED, Zenchak JR, Lossia OV, Hochgeschwender U
Stem cells and development 2018 May 1;27(9):637-647
Stem cells and development 2018 May 1;27(9):637-647
Notch transactivates Rheb to maintain the multipotency of TSC-null cells.
Cho JH, Patel B, Bonala S, Manne S, Zhou Y, Vadrevu SK, Patel J, Peronaci M, Ghouse S, Henske EP, Roegiers F, Giannikou K, Kwiatkowski DJ, Mansouri H, Markiewski MM, White B, Karbowniczek M
Nature communications 2017 Nov 29;8(1):1848
Nature communications 2017 Nov 29;8(1):1848
Analysis of cell growth and gene expression of porcine adipose tissue-derived mesenchymal stem cells as nuclear donor cell.
Oh HJ, Park JE, Park EJ, Kim MJ, Kim GA, Rhee SH, Lim SH, Kang SK, Lee BC
Development, growth & differentiation 2014 Dec;56(9):595-604
Development, growth & differentiation 2014 Dec;56(9):595-604
Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors.
Ohnishi Y, Iwatsuki K, Shinzawa K, Ishihara M, Moriwaki T, Umegaki M, Kishima H, Yoshimine T
Stem cell research 2013 Nov;11(3):1178-90
Stem cell research 2013 Nov;11(3):1178-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody [NBP1-02419] - Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP1-02419] - Staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
