Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 656808 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656808, RRID:AB_2566634
- Product name
- Brilliant Violet 421™ anti-Nestin
- Antibody type
- Monoclonal
- Antigen
- Fusion protein
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Antibody clone number
- 10C2
- Vial size
- 100 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for 30 minutes at room temperature. Then the cells were stained with 1.25 µg/ml of anti-human Nestin (clone 10C2) Brilliant Violet 421™ (magenta) overnight, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes at 4°C. Nuclei were counterstained with DRAQ5 (blue). The image was captured with a 20X objective.
- Conjugate
- Blue dye