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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 656811 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656811, RRID:AB_2616991
- Product name
- Direct-Blot™ HRP anti-Nestin
- Antibody type
- Monoclonal
- Antigen
- Fusion protein
- Reactivity
- Human
- Host
- Mouse
- Antibody clone number
- 10C2
- Vial size
- 100 µl (20 Western blots)
- Storage
- -20°C
- Handling
- Blue Ice
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from NTERA-2 cells (lane 1, 15 µg) and NF-1 cells (lane 2, 15 µg) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with Direct-Blot™ HRP anti-Nestin antibody (clone 10C2). Proteins were visualized with chemiluminescence detection. Direct-Blot™ HRP anti-β-actin antibody (clone 2F1-1) was used as a loading control.
