Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 656812 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#656812, RRID:AB_2629793
- Product name
- Alexa Fluor® 488 anti-Nestin
- Antibody type
- Monoclonal
- Description
- Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Antibody clone number
- 10C2
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for more than one hour at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 488 overnight, followed by Flash Phalloidin™ Red 594 (red) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (purple). The image was captured with a 40X objective.
- Conjugate
- Green dye