656812

antibody from BioLegend

Targeting: NES FLJ21841

Immunocytochemistry (Data presented on provider website)

Immunoelectron microscopy (Supportive data in Antibodypedia)

Antibody Data

Product number

656812

Provider

BioLegend

Proper citation

BioLegend Cat#656812, RRID:AB_2629793

Product name

Alexa Fluor® 488 anti-Nestin

Antibody type

Monoclonal

Description

Nestin is a class VI intermediate filament protein originally found in neuronal stem cells, and is required for survival and renewal of neural progenitor cells. In mixtures, Nestin forms heterodimers with vimentin or internexin, participating in the remodeling of the cell. Nestin was found to be expressed on vascular endothelial progenitor cells and various neoplasms. It is proposed to be a novel angiogenesis marker and cancer therapeutic target.

Reactivity

Human

Host

Mouse

Conjugate

Green dye

Antibody clone number

10C2

Vial size

100 µg

Concentration

0.5 mg/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunoelectron microscopy

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

HUVEC cells were fixed with 1% paraformaldehyde (PFA), permeabilized with 0.5% Triton X-100, and blocked with 5% fetal bovine serum for more than one hour at room temperature. Then the cells were stained with 5 µg/ml of anti-Nestin (clone 10C2) Alexa Fluor® 488 overnight, followed by Flash Phalloidin™ Red 594 (red) staining for 20 minutes at 4°C. Nuclei were counterstained with DAPI (purple). The image was captured with a 40X objective.

Conjugate

Green dye