Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [8]
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Validation data
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- Product number
- NBP2-48504 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Nestin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Nestin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody [NBP2-48504] - Analysis in human cell line RH-30.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-NES antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Nestin Antibody [NBP2-48504] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2,. Remaining relative intensity is presented
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney shows distinct positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining in human kidney and liver tissues using anti-NES antibody. Corresponding NES RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle, kidney, liver and lymph node using Anti-NES antibody NBP2-48504 (A) shows similar protein distribution across tissues to independent antibody NBP2-48575 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human heart muscle.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human kidney using Anti-NES antibody NBP2-48504.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Nestin Antibody [NBP2-48504] - Staining of human liver using Anti-NES antibody NBP2-48504.