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- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [3]
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- Product number
- ABIN152605 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Nestin (NES) (AA 1464-1614) antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Epitope
- AA 1464-1614
- Isotype
- IgG
- Antibody clone number
- 10C2
- Vial size
- 0.1 mL
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
- Handling
- Avoid freeze-thaw cycles
Submitted references Establishment of a tumor sphere cell line from a metastatic brain neuroendocrine tumor.
Activated Notch1 expression is associated with angiogenesis in cutaneous melanoma.
Comprehensive characterization of glioblastoma tumor tissues for biomarker identification using mass spectrometry-based label-free quantitative proteomics.
Nestin and vimentin colocalization affects the subcellular location of glucocorticoid receptor in cutaneous melanoma.
Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
Illumina whole-genome complementary DNA-mediated annealing, selection, extension and ligation platform: assessing its performance in formalin-fixed, paraffin-embedded samples and identifying invasion pattern-related genes in oral squamous cell carcinoma.
Comparative analysis of DNA repair in stem and nonstem glioma cell cultures.
NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity.
Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells.
Gene expression in human neural stem cells: effects of leukemia inhibitory factor.
Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells.
Iwata R, Maruyama M, Ito T, Nakano Y, Kanemura Y, Koike T, Oe S, Yoshimura K, Nonaka M, Nomura S, Sugimoto T, Yamada H, Asai A
Medical molecular morphology 2017 Dec;50(4):211-219
Medical molecular morphology 2017 Dec;50(4):211-219
Activated Notch1 expression is associated with angiogenesis in cutaneous melanoma.
Murtas D, Piras F, Minerba L, Maxia C, Ferreli C, Demurtas P, Lai S, Mura E, Corrias M, Sirigu P, Perra MT
Clinical and experimental medicine 2015 Aug;15(3):351-60
Clinical and experimental medicine 2015 Aug;15(3):351-60
Comprehensive characterization of glioblastoma tumor tissues for biomarker identification using mass spectrometry-based label-free quantitative proteomics.
Heroux MS, Chesnik MA, Halligan BD, Al-Gizawiy M, Connelly JM, Mueller WM, Rand SD, Cochran EJ, LaViolette PS, Malkin MG, Schmainda KM, Mirza SP
Physiological genomics 2014 Jul 1;46(13):467-81
Physiological genomics 2014 Jul 1;46(13):467-81
Nestin and vimentin colocalization affects the subcellular location of glucocorticoid receptor in cutaneous melanoma.
Lai S, Piras F, Spiga S, Perra MT, Minerba L, Piga M, Mura E, Murtas D, Demurtas P, Corrias M, Maxia C, Ferreli C, Sirigu P
Histopathology 2013 Feb;62(3):487-98
Histopathology 2013 Feb;62(3):487-98
Epstein-Barr virus latent membrane protein 1 induces cancer stem/progenitor-like cells in nasopharyngeal epithelial cell lines.
Kondo S, Wakisaka N, Muramatsu M, Zen Y, Endo K, Murono S, Sugimoto H, Yamaoka S, Pagano JS, Yoshizaki T
Journal of virology 2011 Nov;85(21):11255-64
Journal of virology 2011 Nov;85(21):11255-64
Illumina whole-genome complementary DNA-mediated annealing, selection, extension and ligation platform: assessing its performance in formalin-fixed, paraffin-embedded samples and identifying invasion pattern-related genes in oral squamous cell carcinoma.
Loudig O, Brandwein-Gensler M, Kim RS, Lin J, Isayeva T, Liu C, Segall JE, Kenny PA, Prystowsky MB
Human pathology 2011 Dec;42(12):1911-22
Human pathology 2011 Dec;42(12):1911-22
Comparative analysis of DNA repair in stem and nonstem glioma cell cultures.
Ropolo M, Daga A, Griffero F, Foresta M, Casartelli G, Zunino A, Poggi A, Cappelli E, Zona G, Spaziante R, Corte G, Frosina G
Molecular cancer research : MCR 2009 Mar;7(3):383-92
Molecular cancer research : MCR 2009 Mar;7(3):383-92
NK cells recognize and kill human glioblastoma cells with stem cell-like properties.
Castriconi R, Daga A, Dondero A, Zona G, Poliani PL, Melotti A, Griffero F, Marubbi D, Spaziante R, Bellora F, Moretta L, Moretta A, Corte G, Bottino C
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3530-9
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 15;182(6):3530-9
SOX2 silencing in glioblastoma tumor-initiating cells causes stop of proliferation and loss of tumorigenicity.
Gangemi RM, Griffero F, Marubbi D, Perera M, Capra MC, Malatesta P, Ravetti GL, Zona GL, Daga A, Corte G
Stem cells (Dayton, Ohio) 2009 Jan;27(1):40-8
Stem cells (Dayton, Ohio) 2009 Jan;27(1):40-8
Differential processing of amyloid-beta precursor protein directs human embryonic stem cell proliferation and differentiation into neuronal precursor cells.
Porayette P, Gallego MJ, Kaltcheva MM, Bowen RL, Vadakkadath Meethal S, Atwood CS
The Journal of biological chemistry 2009 Aug 28;284(35):23806-17
The Journal of biological chemistry 2009 Aug 28;284(35):23806-17
Gene expression in human neural stem cells: effects of leukemia inhibitory factor.
Wright LS, Li J, Caldwell MA, Wallace K, Johnson JA, Svendsen CN
Journal of neurochemistry 2003 Jul;86(1):179-95
Journal of neurochemistry 2003 Jul;86(1):179-95
Analysis of the temporal expression of nestin in human fetal brain derived neuronal and glial progenitor cells.
Messam CA, Hou J, Berman JW, Major EO
Brain research. Developmental brain research 2002 Mar 31;134(1-2):87-92
Brain research. Developmental brain research 2002 Mar 31;134(1-2):87-92
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Supportive validation
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- Visualization of nestin in PC-3 cells using ABIN152605. Courtesy of Dolnick Lab, Roswell Park Cancer Institute.
Supportive validation
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- IHC
Supportive validation
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- antibodies-online (provider)
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- FACS
- Submitted by
- antibodies-online (provider)
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- FACS
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- FACS
