PA5-11887

antibody from Invitrogen Antibodies

Targeting: NES FLJ21841

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11887

Provider

Invitrogen Antibodies

Product name

Nestin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The invasive proteome of glioblastoma revealed by laser-capture microdissection.

Daubon T, Guyon J, Raymond AA, Dartigues B, Rudewicz J, Ezzoukhry Z, Dupuy JW, Herbert JMJ, Saltel F, Bjerkvig R, Nikolski M, Bikfalvi A
Neuro-oncology advances 2019 May-Dec;1(1):vdz029

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of SY5Y cells using a NES polyclonal antibody (Product # PA5-11887). SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with a NES polyclonal antibody (Product # PA5-11887) (1:200, 2 hr at room temperature). Primary antibody detected by fluor-conjugated donkey anti-rabbit secondary antibody (green) was used (1:1000, 1hr). Nuclei were counterstained with Hoechst 33342 (blue) (10 µg/mL, 5 min).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of HepG2 cells using a NES polyclonal antibody (Product # PA5-11887) (bottom), compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. DNM1 and PLP1 are more expressed in invasive areas of patient-derived xenografts and in patients. (A) Immunostaining of Nestin (red) and DNM1 or PLP1 (green) in the tumor core (upper panels) and in the invasive area (middle panels) of P3 tumors. DAPI was used for nuclear staining (blue). Scale bars: 100 um. Lower panels represent magnified images as delineated by dashed line squares, with plot profiles defined using Fiji software (defined by white lines). Both DNM1 and PLP1 are cytoplasmic. (B) The graphs represent DNM1 and PLP1 staining intensity of 10 different images from P3 core and invasive areas (Student t -test, ** P < .01; *** P < .001). (C) Immunostaining of DNM1 or PLP1 (green) in the tumor core (upper panels) and in the invasive area (lower panels) of P3 tumors. DAPI was used for nuclear staining (blue). Scale bars: 100 um. The graphs represent DNM1 and PLP1 staining intensity of 10 different images of core and invasive areas from several patient sections (Student t -test, *** P < .001; **** P < .0001).