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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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- Product number
- PA5-32517 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Nestin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended prior to staining, using a 1mM EDTA buffer, pH 8.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is liver carcinoma.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- Lot Dependent
- Storage
- 4° C
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Nestin using anti-Nestin Polyclonal Antibody (Product # PA5-32517) in Liver Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:200.
