AP07829PU-N

antibody from Acris Antibodies GmbH

Targeting: NES FLJ21841

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP07829PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP07829PU-N, RRID:AB_1619685

Product name

anti Nestin (1484-1500)

Antibody type

Polyclonal

Antigen

Recombinant protein corresponding to amino acids 1484-1500 of Human Nestin.

Reactivity

Human, Mouse, Rat, Bovine, Canine, Hamster, Rabbit

Host

Rabbit

Vial size

50 µg

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Anti-Nestin Antibody - Western Blot. Western blot using Affinity Purified anti-Nestin antibody shows detection of a band ~220 kD corresponding to mouse Nestin (arrowhead). Approximately 30 g of MEF whole cell lysate was separated by SDS-PAGE using a 4-20% gradient gel. After transfer onto nitrocellulose, the membrane was blocked and then probed with the primary antibody diluted to 1:2000 overnight at 4°C. The membrane was then washed and reacted with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature. IRDye800 fluorescence image was captured using the Ddyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP07829PU-N Nestin antibody staining of Formalin-Fixed Paraffin-Embedded Human Kidney at 5 µg/ml

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

AP07829PU-N Nestin antibody staining of Formalin-Fixed Paraffin-Embedded Mouse Kidney.