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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1259F - Provider product page
- Provider
- R&D Systems
- Product name
- Human Nestin Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Nestin in flow cytometry.
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P48681- Isotype
- IgG
- Antibody clone number
- 196908
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Non integrative strategy decreases chromosome instability and improves endogenous pluripotency genes reactivation in porcine induced pluripotent-like stem cells.
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes.
Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas.
Congras A, Barasc H, Canale-Tabet K, Plisson-Petit F, Delcros C, Feraud O, Oudrhiri N, Hadadi E, Griscelli F, Bennaceur-Griscelli A, Turhan A, Afanassieff M, Ferchaud S, Pinton A, Yerle-Bouissou M, Acloque H
Scientific reports 2016 Jun 1;6:27059
Scientific reports 2016 Jun 1;6:27059
Modeling Smith-Lemli-Opitz syndrome with induced pluripotent stem cells reveals a causal role for Wnt/β-catenin defects in neuronal cholesterol synthesis phenotypes.
Francis KR, Ton AN, Xin Y, O'Halloran PE, Wassif CA, Malik N, Williams IM, Cluzeau CV, Trivedi NS, Pavan WJ, Cho W, Westphal H, Porter FD
Nature medicine 2016 Apr;22(4):388-96
Nature medicine 2016 Apr;22(4):388-96
Arsenic trioxide-mediated Notch pathway inhibition depletes the cancer stem-like cell population in gliomas.
Zhen Y, Zhao S, Li Q, Li Y, Kawamoto K
Cancer letters 2010 Jun 1;292(1):64-72
Cancer letters 2010 Jun 1;292(1):64-72
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Nestin in NTera-2 Human Cell Line by Flow Cytometry. NTera-2 human testicular embryonic carcinoma cell line was stained with Mouse Anti-Human Nestin Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1259F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
