703154

antibody from Invitrogen Antibodies

Targeting: TBK1 NAK

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

703154

Provider

Invitrogen Antibodies

Product name

TBK1 Recombinant Rabbit Monoclonal Antibody (12H60L39)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Antibody clone number

12H60L39

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

The A137R Protein of African Swine Fever Virus Inhibits Type I Interferon Production via the Autophagy-Mediated Lysosomal Degradation of TBK1.

Sun M, Yu S, Ge H, Wang T, Li Y, Zhou P, Pan L, Han Y, Yang Y, Sun Y, Li S, Li LF, Qiu HJ
Journal of virology 2022 May 11;96(9):e0195721

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

For immunofluorescence analysis, MCF-7 and SK-BR-3 cells were fixed and permeabilized for detection of endogenous TBK1 using Anti-TBK1 Recombinant Rabbit Monoclonal Antibody (Product # 703154, 1:100) and labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32731, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:800) was used for cytoskeletal F-actin (red) staining. Panel a-d) clearly demonstrates the nuclear and cytoplasmic localization of TBK1 in MDA-MB-231 cells. Panel e-h) shows reduced specific staining in SK-BR-3 cells which has low expression compared to MDA-MB-231, demonstrating specificity. The images were captured at 60X magnification.