HPA014658

antibody from Atlas Antibodies

Targeting: ARHGEF11 GTRAP48, KIAA0380, PDZ-RHOGEF

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA014658

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA014658, RRID:AB_1850428

Product name

Anti-ARHGEF11

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

FIICTSKLGPPQIYELVALTSSDKNTWMELLEEAV
RNATRHPGAAPMPVHPPPPGPREPAQQGPTPSRVE
LDDSDVFHGEPEPEELPGGTGSQQRVQGKHQVLLE
DPEQEGSAEEEELGVLPCPSTSLDGENRGIR


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and ARHGEF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404959).

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Independent antibody validation

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex, colon, lymph node and testis using Anti-ARHGEF11 antibody HPA014658 (A) shows similar protein distribution across tissues to independent antibody HPA011026 (B).

Antibody #2 product nr

HPA011026, HPA012037

Antibody provider

Atlas Antibodies

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human duodenum shows cytoplasmic positivity in glandular cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebral cortex using Anti-ARHGEF11 antibody HPA014658.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human colon using Anti-ARHGEF11 antibody HPA014658.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis using Anti-ARHGEF11 antibody HPA014658.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node using Anti-ARHGEF11 antibody HPA014658.

Sample type

HUMAN