MA5-15587

antibody from Invitrogen Antibodies

Targeting: RAB25 CATX-8

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15587

Provider

Invitrogen Antibodies

Product name

RAB25 Monoclonal Antibody (3F12)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15587 targets Rab25 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples. The MA5-15587 immunogen is purified recombinant fragment of Rab25 expressed in E. Coli. MA5-15587 detects Rab25 which has a predicted molecular weight of approximately 24kDa.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

3F12

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Rab25 using Rab25 monoclonal antibody (Product # MA5-15587) in MCF-7 (1), T47D (2) and GC7901 (3) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using Rab25 monoclonal antibody (Product # MA5-15587) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of A549 cells using Rab25 monoclonal antibody (Product # MA5-15587) (Green). Blue: DRAQ5 fluorescent DNA dye. Red: actin filaments have been labeled with phalloidin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded esophagus tissues (left) and human lung cancer (right) using Rab25 monoclonal antibody (Product # MA5-15587) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of NIH/3T3 cells using Rab25 monoclonal antibody (Product # MA5-15587) (green) and negative control (purple).