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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
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- Product number
- 66623-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CHST4 Monoclonal Antibody (1E1A4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: DPSLNLHIV HLVRDPRAVF RSRERTKGDL MIDSRIVMGQ HEQKLKKEDQ PYYVMQVICQ SQLEIYKTIQ SLPKALQERY LLVRYEDLAR APVAQTSRMY EFVGLEFLPH LQTWVHNITR GKGMGDHAFH TNARDALNVS QAWRWSLPYE KVSRLQKACG DAMNLLGYRH VRSEQEQRNL LLDLLSTWTV PEQIH (177-386 aa encoded by BC035282)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 1E1A4
- Vial size
- 150 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HT-29 cells were subjected to SDS PAGE followed by western blot with 66623-1-IG (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 66623-1-Ig (CHST4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using (Product # 66623-1-IG) (CHST4 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human liver tissue slide using 66623-1-Ig (CHST4 antibody) at dilution of 1:200 (under 40x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66623-1-IG (CHST4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human kidney tissue slide using 66623-1-IG (CHST4 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
