PA5-72551

antibody from Invitrogen Antibodies

Targeting: NR1I2 BXR, ONR1, PAR2, PXR, SXR

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-72551

Provider

Invitrogen Antibodies

Product name

PXR Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Indolepropionic Acid, a Metabolite of the Microbiome, Has Cytostatic Properties in Breast Cancer by Activating AHR and PXR Receptors and Inducing Oxidative Stress.

Sári Z, Mikó E, Kovács T, Jankó L, Csonka T, Lente G, Sebő É, Tóth J, Tóth D, Árkosy P, Boratkó A, Ujlaki G, Török M, Kovács I, Szabó J, Kiss B, Méhes G, Goedert JJ, Bai P
Cancers 2020 Aug 25;12(9)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NR1I2 in HL-60 cell lysate using 35 µg of protein per lane. Samples were incubated with NR1I2 polyclonal antibody (Product # PA5-72551).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NR1I2 in HL-60 cell lysate using 35 µg of protein per lane. Samples were incubated with NR1I2 polyclonal antibody (Product # PA5-72551).

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of NR1I2 in HepG2 cells using confocal microscopy. Sample was incubated with NR1I2 polyclonal antibody (Product # PA5-72551) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) secondary antibody. Actin filaments were labeled with Alexa Fluor 555 Phalloidin (red) and DAPI was used to stain the cell nuclei (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry analysis of NR1I2 in HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with NR1I2 polyclonal antibody (Product # PA5-72551) followed by a FITC conjugated goat anti-rabbit secondary antibody.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 8 Higher intratumoral expression of pregnane X receptor (PXR) and nuclear aryl hydrocarbon receptor (AHR) shows correlation with low-grade and lower-mitosis breast cancers. ( A ) TMA was stained with the indicated antibodies. A typical staining pattern is shown. The bar is equivalent of 50 um. Cases in TMA were scored for receptor expression using the H-score system. ( B - E ) H-score of the nuclear expression of the AHR receptor were related to ( B ) stage of the disease, ( C ) mitosis score, ( D ) tubule formation, and ( E ) histological subtype. ( F - H ) H-score of the expression of PXR receptor were related to ( F ) grade of the disease, ( G ) mitosis score, and ( H ) mitotic index. Statistical significance on panels B and F was determined using an ANOVA test followed by Dunnett's post-hoc test, while on panels C, D, E, G, and H, Student's t -test was used. Stage 0 (in situ carcinoma) and stage 1 patients, Mitosis score 2 and 3 patients, and tubule formation score 1 and 2 patients were handled together due to the low number of cases. Abbreviations: Aryl hydrocarbon receptor (AHR), Pregnane X receptor (PXR), no special type (NOS). *, ** and *** indicate statistically significant difference between control and treated samples at p < 0.05, p < 0.01 or p < 0.001, respectively.