AF1339

LGALS7 antibody from R&D Systems

GAL7, LGALS7A, PIG1, TP53I1

Eligible for validation within the Antibodypedia Validation Initiative

Western blot

Supportive data in Antibodypedia

ELISA

Data presented on provider website

Immunohistochemistry

Data presented on provider website

Provider product page for AF1339

SELECT THIS
ANTIBODY

PERFORM KNOCKDOWN
OR KNOCKOUT

SUBMIT POSITIVE
RESULTS

GET
REWARDED

Antibody Data

Product number

AF1339

Provider

R&D Systems

Product name

Human Galectin-7 Antibody

Provider product page

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human Galectin-7 in ELISAs and Western blots. In sandwich immunoassays, approximately 50% cross-reactivity with recombinant mouse (rm) Galectin-7 is observed and less than 0.5% cross-reactivity with recombinant human (rh) Galectin-1, rmGalectin-3, rhGalectin-4, and rhGalectin-8 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

NP_002298


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Tissue and plasma levels of galectins in patients with high grade serous ovarian carcinoma as new predictive biomarkers.
Labrie M, De Araujo LOF, Communal L, Mes-Masson AM, St-Pierre Y
Scientific reports 2017 Oct 16;7(1):13244

---

A Mutation in the Carbohydrate Recognition Domain Drives a Phenotypic Switch in the Role of Galectin-7 in Prostate Cancer.
Labrie M, Vladoiu M, Leclerc BG, Grosset AA, Gaboury L, Stagg J, St-Pierre Y
PloS one 2015;10(7):e0131307

---

The CCAAT/enhancer-binding protein beta-2 isoform (CEBPβ-2) upregulates galectin-7 expression in human breast cancer cells.
Campion CG, Labrie M, Grosset AA, St-Pierre Y
PloS one 2014;9(5):e95087

---

Galectin-7 acts as an adhesion molecule during implantation and increased expression is associated with miscarriage.
Menkhorst EM, Gamage T, Cuman C, Kaitu'u-Lino TJ, Tong S, Dimitriadis E
Placenta 2014 Mar;35(3):195-201

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Galectin-7 by Western Blot. Western blot shows lysates of human skin tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-7 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Galectin-7 by Simple Western<sup abp="265">TM. Simple Western lane view shows lysates of HEK001 human epidermal keratinocyte cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-7 at approximately 21 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1339) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.