AHC7129

antibody from Invitrogen Antibodies

Targeting: IL12B CLMF, CLMF2, IL-12B, NKSF, NKSF2

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

AHC7129

Provider

Invitrogen Antibodies

Product name

IL-12 p70 Monoclonal Antibody (A25C4B6), Biotin

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

This product is recommended as a detection antibody in Sandwich ELISA applications. This clone can also be referred to as clone number A25C 4B6 1B3.

Reactivity

Human

Host

Mouse

Conjugate

Biotin

Isotype

IgG

Antibody clone number

A25C4B6

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Increased plasma levels of IL-12 and IL-6 correlate with increased non-classical CD16 bright CD14 neg monocytes as well as with increased activation of classical CD16 neg CD14 bright monocytes in patients with MDD and suicide behaviour. Plasma levels of (a) IL-12p70 and (b) IL-6 in patients with MDD (n=33) and healthy controls (n=20) as determined by ELISA. Significant differences between patients with MDD and healthy controls were calculated using a 2-tailed Mann-Whitney test (** p < 0.01). (c) Representative dot plots, after gating in mononuclear CD11b + cells, showing the frequency of the three monocyte subsets based on CD14 vs. CD16 expression level in healthy controls and patients with MDD. Detailed gaiting strategy is shown in Supplementary Fig. S1. (d) The three monocytes subset are depicted as classical CD16 neg CD14 bright (blue), non-classical CD16 bright CD14 neg (green) and intermediate CD16 + CD14 + (red) for further analysis. Independent data are graphed in (e-g) showing decreased percentages of classical CD16 neg CD14 bright monocytes alongside an increased percentage of non-classical CD16brightCD14neg monocytes in peripheral blood of patients with MDD. The frequency of the three subpopulations of monocytes from the cohort of patients with MDD was also segregated based on IL-6 and IL-12 plasma levels. The threshold for high cytokine level was set as the mean value plus 1 SD of control group. One-way ANOVA test was performed (** p < 0.01; ns p > 0.05). (h

Conjugate

Biotin