Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF1954 - Provider product page
- Provider
- R&D Systems
- Product name
- Feline IL-12/IL-23 p40 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects feline IL-12/IL-23 p40 in ELISAs and Western blots. In sandwich immunoassays, approximately 65% cross-reactivity with recombinant canine IL-12/23 p40 is observed, less than 3% cross-reactivity with recombinant mouse IL-12/23 p40 is observed, and less than 0.2% cross-reactivity with recombinant human IL-12/23 p40 is observed.
- Reactivity
- Feline
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O02744
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Efficient plant-based production of chicken interleukin-12 yields a strong immunostimulatory cytokine.
Medrano G, Dolan MC, Stephens NT, McMickle A, Erf G, Radin D, Cramer CL
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2010 Mar;30(3):143-54
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research 2010 Mar;30(3):143-54
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-12/IL-23 p40 in Feline PBMCs. IL-12/IL-23 p40 was detected in immersion fixed feline peripheral blood mononuclear cells (PBMCs) stimulated with PMA and calcium ionomycin using Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-12 and Neutralization by Feline IL-12/IL-23 p40 Antibody. Recombinant Feline IL-12 (Catalog # 1954-FL) stimulates proliferation in PHA-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Feline IL-12 (25 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Feline IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1954). The ND50 is typically 0.5-2.0 µg/mL.