MAB4991-100

antibody from R&D Systems

Targeting: IL12B CLMF, CLMF2, IL-12B, NKSF, NKSF2

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB4991-100

Provider

R&D Systems

Product name

Mouse IL-12/IL-23 p40 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects the mouse IL-12/IL-23 p40 subunit. Polymorphisms exist in the mouse p40 sequences [Ymer, S. et al. (2002) Genes and Immunity 3:151.].

Reactivity

Mouse

Host

Rat

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

C17.8

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

IFN-γ-Expressing Th17 Cells Are Required for Development of Severe Ocular Surface Autoimmunity.

Chen Y, Chauhan SK, Shao C, Omoto M, Inomata T, Dana R
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 1;199(3):1163-1169

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M2b macrophage elimination and improved resistance of mice with chronic alcohol consumption to opportunistic infections.

Ohama H, Asai A, Ito I, Suzuki S, Kobayashi M, Higuchi K, Suzuki F
The American journal of pathology 2015 Feb;185(2):420-31

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Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.

DePaolo RW, Abadie V, Tang F, Fehlner-Peach H, Hall JA, Wang W, Marietta EV, Kasarda DD, Waldmann TA, Murray JA, Semrad C, Kupfer SS, Belkaid Y, Guandalini S, Jabri B
Nature 2011 Mar 10;471(7337):220-4

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The pro-Th2 cytokine IL-33 directly interacts with invariant NKT and NK cells to induce IFN-gamma production.

Bourgeois E, Van LP, Samson M, Diem S, Barra A, Roga S, Gombert JM, Schneider E, Dy M, Gourdy P, Girard JP, Herbelin A
European journal of immunology 2009 Apr;39(4):1046-55

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TSU68 prevents liver metastasis of colon cancer xenografts by modulating the premetastatic niche.

Yamamoto M, Kikuchi H, Ohta M, Kawabata T, Hiramatsu Y, Kondo K, Baba M, Kamiya K, Tanaka T, Kitagawa M, Konno H
Cancer research 2008 Dec 1;68(23):9754-62

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Glechoma hederacea inhibits inflammatory mediator release in IFN-gamma and LPS-stimulated mouse peritoneal macrophages.

An HJ, Jeong HJ, Um JY, Kim HM, Hong SH
Journal of ethnopharmacology 2006 Jul 19;106(3):418-24

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17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.

Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood 2004 Sep 1;104(5):1404-10

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Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12.

Oppmann B, Lesley R, Blom B, Timans JC, Xu Y, Hunte B, Vega F, Yu N, Wang J, Singh K, Zonin F, Vaisberg E, Churakova T, Liu M, Gorman D, Wagner J, Zurawski S, Liu Y, Abrams JS, Moore KW, Rennick D, de Waal-Malefyt R, Hannum C, Bazan JF, Kastelein RA
Immunity 2000 Nov;13(5):715-25

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-12 and Neutralization by Mouse IL-12/IL-23 p40 Antibody. Recombinant Mouse IL-12 (Catalog # 419-ML) stimulates proliferation in PHA-activated mouse splenocytes in a dose-dependent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Mouse IL-12 (1 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-12/IL-23 p40 Monoclonal Antibody (Catalog # MAB4991). The ND₅₀ is typically 0.04-0.3 µg/mL.