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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 501816 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#501816, RRID:AB_893537
- Product name
- Alexa Fluor® 488 anti-human IL-12/IL-23 p40 [C11.5]
- Antibody type
- Monoclonal
- Antigen
- CHO-expressed, recombinant human IL-12 p70 heterod
- Description
- The C11.5 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C11.5 antibody can neutralize the bioactivity of natural or recombinant IL-12 p70.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Vial size
- 100 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- IFN-g-primed and then LPS-stimulated (overnight) human peripheral blood monocytes surface stained with with CD14 (M5E2) PE and intracellular stained with mouse IgG1 Alexa Fluor® 488 isotype control (upper panel) or C11.5 Alexa Fluor® 488 (lower panel)
- Conjugate
- Green dye
