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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- AP52183PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP52183PU-N, RRID:AB_11144603
- Product name
- anti Interleukin-12 beta / IL12B (C-term)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide - KLH conjugated - corresponding to the C-terminal region (between 271-298aa) of human Interleukin-12 beta/IL12B.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.4 ml
- Concentration
- lot specific
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis in CEM,MDA-MB453 cell line lysates (35ug/lane) using Interleukin-12 beta/IL12B antibody Cat.-No. AP52183PU-N (C-term). This demonstrates the IL12B antibody detected the IL12B protein (arrow).
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in human tonsil tissue (formalin-fixed, paraffin-embedded) using Interleukin-12 beta/IL12Bantibody Cat.-No. AP52183PU-N (C-term), followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the IL12B antibody (C-term) for IHC. Clinical relevance has not been evaluated.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram) using Interleukin-12 beta/IL12B antibody Cat.-No. AP52183PU-N (C-term), followed by FITC-conjugated goat-anti-rabbit secondary antibodies.
