Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF309 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-12/IL-23 p40 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-12/IL-23 p40 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human IL-23 p19 and recombinant mouse IL-23 p19 was observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Clustering of disulfide-rich peptides provides scaffolds for hit discovery by phage display: application to interleukin-23.
Barkan DT, Cheng XL, Celino H, Tran TT, Bhandari A, Craik CS, Sali A, Smythe ML
BMC bioinformatics 2016 Nov 23;17(1):481
BMC bioinformatics 2016 Nov 23;17(1):481
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-12/IL-23 p40 by Western Blot. Western blot shows lysates of Hs 294T human melanoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-12/IL-23 p40 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF309) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-12/IL-23 p40 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Il-17 Secretion Induced by IL-23 and Neutralization by Human IL-12/IL-23 p40 Antibody. Recombinant Human IL-23 (Catalog # 1290-IL) induces IL-17 secretion in the mouse splenocytes in a dose-dependent manner (orange line), as measured by Mouse IL-17 Quantikine kit. IL-17 Secretion elicited by Recombinant Human IL-23 (0.75 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-12/IL-23 p40 Anti-gen Affinity-purified Polyclonal Antibody (Catalog # AF309). The ND50 is typically 0.004-0.02 μg/mL.