56-0038-41

antibody from Invitrogen Antibodies
Targeting: CD247 CD3H, CD3Q, CD3Z
Provider product page for 56-0038-41

Antibody data

Product number
56-0038-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD3 Monoclonal Antibody (UCHT1), Alexa Fluor 700, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHT1 antibody is offered in 2 formats: - µg size: has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The Alexa Fluor® 700 emits at 723 nm and can be excited with the red laser (633 mm). Most instruments will require a 685 LP mirror and 710/20 filter. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 633-647 nm; Emission: 723 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Conjugate
Near infrared dye
Isotype
IgG
Antibody clone number
UCHT1
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
CD247 protein structure - 56-0038-41 shown in red.