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- References [21]
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- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- 13520-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13520-1-AP, RRID:AB_2079160
- Product name
- Choline Kinase Alpha antibody
- Antibody type
- Polyclonal
- Description
- KD/KO validated Choline Kinase Alpha antibody (Cat. #13520-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, IP, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis.
Altered choline level in atherosclerotic lesions: Upregulation of choline transporter-like protein 1 in human coronary unstable plaque.
Functional roles of FAP-α in metabolism, migration and invasion of human cancer cells.
Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.
Twist alters the breast tumor microenvironment via choline kinase to facilitate an aggressive phenotype.
The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.
DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.
Crosstalk of FGFR1 signaling and choline metabolism promotes cell proliferation and survival in prostate cancer cells.
1,25(OH)(2)D(3) alleviates LPS-induced preeclampsia-like rats impairment in the protective effect by TLR4/NF-kB pathway.
Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.
Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.
Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis.
Investigation of discriminant metabolites in tamoxifen-resistant and choline kinase-alpha-downregulated breast cancer cells using 1H-nuclear magnetic resonance spectroscopy.
Preclinical Development of a Nontoxic Oral Formulation of Monoethanolamine, a Lipid Precursor, for Prostate Cancer Treatment.
Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice.
The Tumor Microenvironment Modulates Choline and Lipid Metabolism.
Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells.
Choline kinase inhibition in rheumatoid arthritis.
Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival.
Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.
ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.
Zhang F, Guo L, Shi J, Jiang H, Zhou F, Zhou Y, Lv B, Xu M
Neurobiology of disease 2024 Feb;191:106390
Neurobiology of disease 2024 Feb;191:106390
Altered choline level in atherosclerotic lesions: Upregulation of choline transporter-like protein 1 in human coronary unstable plaque.
Nakamura E, Maekawa K, Saito Y, Matsumoto T, Ogawa M, Komohara Y, Asada Y, Yamashita A
PloS one 2023;18(2):e0281730
PloS one 2023;18(2):e0281730
Functional roles of FAP-α in metabolism, migration and invasion of human cancer cells.
Mori N, Jin J, Krishnamachary B, Mironchik Y, Wildes F, Vesuna F, Barnett JD, Bhujwalla ZM
Frontiers in oncology 2023;13:1068405
Frontiers in oncology 2023;13:1068405
Novel function of Roxadustat (FG-4592) as an anti-shock drug in sepsis by regulating mitochondrial oxidative stress and energy metabolism.
Long G, Pei Z, Wu M, Wei K, Du Y, Wang Q, Zhang Y, Huang S, Chen H, Xia W, Jia Z
Biochimica et biophysica acta. General subjects 2023 Jan;1867(1):130264
Biochimica et biophysica acta. General subjects 2023 Jan;1867(1):130264
Twist alters the breast tumor microenvironment via choline kinase to facilitate an aggressive phenotype.
Vesuna F, Penet MF, Mori N, Bhujwalla ZM, Raman V
Molecular and cellular biochemistry 2023 Apr;478(4):939-948
Molecular and cellular biochemistry 2023 Apr;478(4):939-948
The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation.
Ma Q, Jiang H, Ma L, Zhao G, Xu Q, Guo D, He N, Liu H, Meng Z, Liu J, Zhu L, Lin Q, Wu X, Li M, Luo S, Fang J, Lu Z
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 11;120(15):e2209435120
Proceedings of the National Academy of Sciences of the United States of America 2023 Apr 11;120(15):e2209435120
DHRS2 inhibits cell growth and metastasis in ovarian cancer by downregulation of CHKα to disrupt choline metabolism.
Li Z, Tan Y, Li X, Quan J, Bode AM, Cao Y, Luo X
Cell death & disease 2022 Oct 3;13(10):845
Cell death & disease 2022 Oct 3;13(10):845
Crosstalk of FGFR1 signaling and choline metabolism promotes cell proliferation and survival in prostate cancer cells.
Fan Z, Ma J, Pan X, Zhao L, Wu Y, Lin H, Zhao Y, Jiang H, Pan T, Li X, Wang F, Wang C
International journal of cancer 2022 May 1;150(9):1525-1536
International journal of cancer 2022 May 1;150(9):1525-1536
1,25(OH)(2)D(3) alleviates LPS-induced preeclampsia-like rats impairment in the protective effect by TLR4/NF-kB pathway.
Ma Y, Yang Y, Lv M, Zhang Y, He Q, Zhang Y, Su H, Deng X, Qian Y
Placenta 2022 Dec;130:34-41
Placenta 2022 Dec;130:34-41
Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.
Kong M, Dong W, Xu H, Fan Z, Miao X, Guo Y, Li C, Ye Q, Wang Y, Xu Y
Frontiers in cell and developmental biology 2021;9:705302
Frontiers in cell and developmental biology 2021;9:705302
Choline Uptake and Metabolism Modulate Macrophage IL-1β and IL-18 Production.
Sanchez-Lopez E, Zhong Z, Stubelius A, Sweeney SR, Booshehri LM, Antonucci L, Liu-Bryan R, Lodi A, Terkeltaub R, Lacal JC, Murphy AN, Hoffman HM, Tiziani S, Guma M, Karin M
Cell metabolism 2019 Jun 4;29(6):1350-1362.e7
Cell metabolism 2019 Jun 4;29(6):1350-1362.e7
Nuclear lipid droplets derive from a lipoprotein precursor and regulate phosphatidylcholine synthesis.
Sołtysik K, Ohsaki Y, Tatematsu T, Cheng J, Fujimoto T
Nature communications 2019 Jan 28;10(1):473
Nature communications 2019 Jan 28;10(1):473
Investigation of discriminant metabolites in tamoxifen-resistant and choline kinase-alpha-downregulated breast cancer cells using 1H-nuclear magnetic resonance spectroscopy.
Kim HS, Tian L, Kim H, Moon WK
PloS one 2017;12(6):e0179773
PloS one 2017;12(6):e0179773
Preclinical Development of a Nontoxic Oral Formulation of Monoethanolamine, a Lipid Precursor, for Prostate Cancer Treatment.
Saxena R, Yang C, Rao M, Turaga RC, Garlapati C, Gundala SR, Myers K, Ghareeb A, Bhattarai S, Kamalinia G, Bristi S, Su D, Gadda G, Rida PCG, Cantuaria GH, Aneja R
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jul 15;23(14):3781-3793
Clinical cancer research : an official journal of the American Association for Cancer Research 2017 Jul 15;23(14):3781-3793
Liquid chromatography mass spectrometry-based profiling of phosphatidylcholine and phosphatidylethanolamine in the plasma and liver of acetaminophen-induced liver injured mice.
Ming YN, Zhang JY, Wang XL, Li CM, Ma SC, Wang ZY, Liu XL, Li XB, Mao YM
Lipids in health and disease 2017 Aug 14;16(1):153
Lipids in health and disease 2017 Aug 14;16(1):153
The Tumor Microenvironment Modulates Choline and Lipid Metabolism.
Mori N, Wildes F, Takagi T, Glunde K, Bhujwalla ZM
Frontiers in oncology 2016;6:262
Frontiers in oncology 2016;6:262
Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells.
Kim HS, Tian L, Jung M, Choi SK, Sun Y, Kim H, Moon WK
PloS one 2015;10(10):e0141110
PloS one 2015;10(10):e0141110
Choline kinase inhibition in rheumatoid arthritis.
Guma M, Sanchez-Lopez E, Lodi A, Garcia-Carbonell R, Tiziani S, Karin M, Lacal JC, Firestein GS
Annals of the rheumatic diseases 2015 Jul;74(7):1399-407
Annals of the rheumatic diseases 2015 Jul;74(7):1399-407
Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival.
Mori N, Wildes F, Kakkad S, Jacob D, Solaiyappan M, Glunde K, Bhujwalla ZM
NMR in biomedicine 2015 Dec;28(12):1697-706
NMR in biomedicine 2015 Dec;28(12):1697-706
Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.
Aitchison AJ, Arsenault DJ, Ridgway ND
Molecular biology of the cell 2015 Aug 15;26(16):2927-38
Molecular biology of the cell 2015 Aug 15;26(16):2927-38
ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.
Arsenault DJ, Yoo BH, Rosen KV, Ridgway ND
The Journal of biological chemistry 2013 Jan 4;288(1):633-43
The Journal of biological chemistry 2013 Jan 4;288(1):633-43
No comments: Submit comment
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP(CHKA antibody) at dilution of 1:300
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human liver using 13520-1-AP(CHKA antibody) at dilution of 1:50 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- The Choline kinase alpha antibody from Proteintech is a rabbit polyclonal antibody to a recombinant protein of human Choline kinase alpha. This antibody recognizes human,mouse,rat antigen. The Choline kinase alpha antibody has been validated for the following applications: ELISA, WB, IHC analysis.
