PA5-54995

antibody from Invitrogen Antibodies

Targeting: CHKA CHK, CKI

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-54995

Provider

Invitrogen Antibodies

Product name

CHKA Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: FDIGNHFCEW MYDYSYEKYP FFRANIRKYP TKKQQLHFIS SYLPAFQNDF ENLSTEEKSI IKEEMLLEVN RFALASHFLW GLWSIVQAKI S

Concentration

0.1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHKA in control (vector only transfected HEK293T lysate) and CHKA over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a CHKA Polyclonal Antibody (Product # PA5-54995).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of CHKA in human cell lines SK-MEL-30 and U-251MG using a CHKA Polyclonal Antibody (Product # PA5-54995). Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: Anti-HDAC1.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of CHKA in human testis tissue shows moderate cytoplasmic and nuclear positivity in cells in seminiferous ducts. Samples were probed using a CHKA Polyclonal Antibody (Product # PA5-54995).