13520-1-AP

antibody from Invitrogen Antibodies

Targeting: CHKA CHK, CKI

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

13520-1-AP

Provider

Invitrogen Antibodies

Product name

Choline kinase alpha Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: EPRKVLLRL YGAILQVGAE AMVLESVMFA ILAERSLGPK LYGIFPQGRL EQFIPSRRLD TEELGLPDIS AEIAEKMATF HGMKMPFNKE PKWLFGTMEK YLKEVLRIKF TEESRIKKLH KLLSYNLPLE LENLRSLLES TPSPVVFCHN DCQEGNILLL EGRENSEKQK LMLIDFEYSS YNYRGFDIGN HFCEWMYDYS YEKYPFFRAN IRKYPTKKQQ LHFISSYLPA FQNDFENLST EEKSIIKEEM LLEVNRFALA SHFLWGQWSI VQAKISSIEF GYMDYAQARF DAYFHQKRKL GV (139-439 aa encoded by BC036471)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.26 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse colon tissue were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using (Product # 13520-1-AP) (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-Choline kinase alpha (IP:13520-1-AP, 4ug; Detection:13520-1-AP 1:500) with mouse spleen tissue lysate 4000ug.