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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX23516 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23516, RRID:AB_385084
- Product name
- Calsequestrin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit, Sheep
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Western blot analysis of Calsequestrin in 25 μg of RD, L6 and mouse heart lysates. Proteins were transferred to a PVDF membrane and blocked at 4°C overnight. The membrane was probed with Calsequestrin antibody at a dilution of 1:5000 overnight at 4°C, washed in TBST, and probed with an HRP-conjμgated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Calsequestrin (green) in C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with Calpastatin antibody [2G11D6] in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a proper secondary antibody. F-actin (red) was stained with a flourescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of Calsequestrin in paraffin-treated human heart tissue (right) compared with a negative control in the absence of primary antibody (left). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with Calsequestrin antibody diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively PBST and detection was performed using an HRP-conjμgated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
