Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-88181 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88181, RRID:AB_11026114
- Product name
- Rabbit Polyclonal Calsequestrin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Calsequestrin 1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model.
Kato BS, Nicholson G, Neiman M, Rantalainen M, Holmes CC, Barrett A, Uhlén M, Nilsson P, Spector TD, Schwenk JM
Proteome science 2011 Nov 17;9:73
Proteome science 2011 Nov 17;9:73
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Calsequestrin 1 Antibody [NBP1-88181] - Analysis in control (vector only transfected HEK293T lysate) and CASQ1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining of human heart muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Calsequestrin 1 Antibody [NBP1-88181] - Staining in human skeletal muscle and heart muscle tissues using anti-CASQ1 antibody. Corresponding CASQ1 RNA-seq data are presented for the same tissues.