PAB29356

antibody from Abnova Corporation

Targeting: FHL1 bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29356

Provider

Abnova Corporation

Product name

FHL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human FHL1.

Antigen sequence

KFCANTCVECRKPIGADSKEVHYKNRFWHDTCFRC
AKCLHPLANETFVAKDNKILCNKCTTREDSPKCKG
CFKAIVAGDQNVEYKGTVWHKDCFTCSNCKQV


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with FHL1 polyclonal antibody (Cat # PAB29356) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-251 MG with FHL1 polyclonal antibody (Cat # PAB29356) at 1-4 ug/mL concentration shows positivity in cytoplasm.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle with FHL1 polyclonal antibody (Cat # PAB29356) shows strong nuclear and cytoplasmic positivity in myocytes at 1:200-1:500 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)