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LearnPAB6686
antibody from Abnova Corporation
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB6686 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB6686, RRID:AB_1574876
- Product name
- FHL1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of FHL1.
- Antigen sequence
CNKRFVFHNEQVY- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of two isoforms of the skeletal muscle LIM protein 1, SLIM1. Localization of SLIM1 at focal adhesions and the isoform slimmer in the nucleus of myoblasts and cytoplasm of myotubes suggests distinct roles in the cytoskeleton and in nuclear-cytoplasmic communication.
Brown S, McGrath MJ, Ooms LM, Gurung R, Maimone MM, Mitchell CA
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
The Journal of biological chemistry 1999 Sep 17;274(38):27083-91
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FHL1 polyclonal antibody (Cat # PAB6686) staining (0.05 ug/mL) of human muscle lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- FHL1 polyclonal antibody (Cat # PAB6686, 2.5 ug/mL) staining of paraffin embedded human testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
