TA501279

antibody from OriGene

Targeting: FHL1 bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501279

Provider

OriGene

Proper citation

OriGene Cat#TA501279, RRID:AB_11141760

Product name

FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)

Antibody type

Monoclonal

Description

FHL1 mouse monoclonal antibody, clone OTI2E11 (formerly 2E11)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

FHL1

Isotype

IgG

Antibody clone number

OTI2E11

Vial size

100 µl

Concentration

0.71 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-FHL1 mouse monoclonal antibody (TA501279) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501279), and then analyzed by flow cytometry.

Validation comment

FC