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LearnTA501375
antibody from OriGene
Targeting: FHL1
bA535K18.1, FHL1B, FLH1A, KYO-T, MGC111107, SLIM1, XMPMA
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Flow cytometry [1]
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Validation data
Reference
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- Product number
- TA501375 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501375, RRID:AB_11127557
- Product name
- Anti-FHL1 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)
- Antibody type
- Monoclonal
- Description
- Anti-FHL1 mouse monoclonal antibody, clone OTI3H3 (formerly 3H3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FHL1
- Isotype
- IgG
- Antibody clone number
- OTI3H3
- Vial size
- 100 µl
- Concentration
- 0.68 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FHL1 (RC203478, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FHL1.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FHL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FHL1 mouse monoclonal antibody (TA501375) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FHL1(RC203478).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC203478 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-FHL1 antibody(TA501375), and then analyzed by flow cytometry.
- Validation comment
- FC
